RT Journal Article
T1 Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells.
A1 Garzón, Ingrid
A1 Jaimes-Parra, Boris Damián
A1 Pascual-Geler, Manrique
A1 Cózar, José Manuel
A1 Sánchez-Quevedo, María Del Carmen
A1 Mosquera-Pacheco, María Auxiliadora
A1 Sánchez-Montesinos, Indalecio
A1 Fernández-Valadés, Ricardo
A1 Campos, Fernando
A1 Alaminos, Miguel
K1 biofabrication
K1 human Wharton jelly mesenchymal stromal cells
K1 urothelial mucosa
AB Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin-agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.
YR 2021
FD 2021-05-13
LK https://hdl.handle.net/10668/28138
UL https://hdl.handle.net/10668/28138
LA en
DS RISalud
RD Apr 11, 2025