RT Journal Article
T1 Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats
A1 Casuso, Rafael A.
A1 Al Fazazi, Saad
A1 Plaza-Diaz, Julio
A1 Ruiz-Ojeda, Francisco J.
A1 Rueda-Robles, Ascension
A1 Aragon-Vela, Jeronimo
A1 Huertas, Jesus R.
K1 polyphenols
K1 mitochondria
K1 glycolysis
K1 gene expression
K1 Virgin olive oil
K1 Mitochondrial
K1 Mct1
K1 Antioxidant
K1 Population
K1 Increases
K1 Reveals
K1 Heart
AB We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content.
PB Mdpi
YR 2021
FD 2021-12-01
LK https://hdl.handle.net/10668/26193
UL https://hdl.handle.net/10668/26193
LA en
DS RISalud
RD Apr 17, 2025