%0 Journal Article
%A Casuso, Rafael A.
%A Al Fazazi, Saad
%A Plaza-Diaz, Julio
%A Ruiz-Ojeda, Francisco J.
%A Rueda-Robles, Ascension
%A Aragon-Vela, Jeronimo
%A Huertas, Jesus R.
%T Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats
%D 2021
%U https://hdl.handle.net/10668/26193
%X We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content.
%K polyphenols
%K mitochondria
%K glycolysis
%K gene expression
%K Virgin olive oil
%K Mitochondrial
%K Mct1
%K Antioxidant
%K Population
%K Increases
%K Reveals
%K Heart
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