RT Journal Article
T1 A Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in rams.
A1 Muñoz, Pilar M
A1 Conde-Álvarez, Raquel
A1 Andrés-Barranco, Sara
A1 de Miguel, María-Jesús
A1 Zúñiga-Ripa, Amaia
A1 Aragón-Aranda, Beatriz
A1 Salvador-Bescós, Miriam
A1 Martínez-Gómez, Estrella
A1 Iriarte, Maite
A1 Barberán, Montserrat
A1 Vizcaíno, Nieves
A1 Moriyón, Ignacio
A1 Blasco, José M
K1 B. ovis
K1 Brucella
K1 Sheep
K1 brucellosis
K1 rough
K1 vaccine
AB Brucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. B. melitensis carries a smooth (S) lipopolysaccharide (LPS) with an N-formyl-perosamine O-polysaccharide (O-PS) that is absent in the rough LPS of B. ovis. Their control and eradication require vaccination, but B. melitensis Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once B. melitensis is eradicated or where it never existed, hampering B. ovis control and eradication. To develop a B. ovis specific vaccine, we investigated three Brucella live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CAΔwadB (CO2-independent B. ovis with truncated LPS core oligosaccharide); Rev1::wbdRΔwbkC (carrying N-acetylated O-PS); and H38ΔwbkF (B. melitensis rough mutant with intact LPS core). After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. H38ΔwbkF yielded similar protection to Rev 1 against B. ovis but Bov::CAΔwadB and Rev1::wbdRΔwbkC conferred no or poor protection, respectively. All H38ΔwbkF vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation B. ovis diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for B. melitensis diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38ΔwbkF is an interesting candidate for the immunoprophylaxis of B. ovis in B. melitensis-free areas.
YR 2022
FD 2022-03-02
LK http://hdl.handle.net/10668/20365
UL http://hdl.handle.net/10668/20365
LA en
DS RISalud
RD Apr 9, 2025