RT Journal Article
T1 Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?
A1 Goikoetxea, M J
A1 D'Amelio C M, 
A1 Martínez-Aranguren, R
A1 Gamboa, P
A1 Garcia, B E
A1 Gómez, F
A1 Fernández J, 
A1 Bartra, J
A1 Parra, A
A1 Alvarado, M I
A1 Alonso, M I
A1 González, E
A1 Terrados, S
A1 Moya, C
A1 Blanca, N
A1 Feo-Brito, F
A1 Villalba, M
A1 Díaz-Perales, A
A1 Sanz, M L
K1 sIgE
K1 ISAC
K1 ImmunoCAP
K1 LTP
K1 Nut allergy
AB Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy.Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy.Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cora 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges.Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P < .05). Similar rates of Cora 8 and Jug r 3 sensitization were detected by both techniques.Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.
PB JIACI
SN 1018-9068
YR 2016
FD 2016
LK https://hdl.handle.net/10668/27794
UL https://hdl.handle.net/10668/27794
LA en
NO Goikoetxea MJ, D'Amelio C M, Martínez-Aranguren R, Gamboa P, Garcia BE, Gómez F, et al. Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?. J Investig Allergol Clin Immunol. 2016;26(1):31-39.
NO This research was financially supported by Instituto de Salud Carlos III (Grant: PI 11/01634) and by grant RD12/0013/00010 from the Spanish Research Network on Adverse Reactions to Allergens and Drugs (RIRAAF: Red de Investigación de Reacciones Adversas a Alérgenos y Fármacos) of the Carlos III Health Institute.
DS RISalud
RD Oct 5, 2025