%0 Journal Article
%A Gomez-Gonzalez, Emilio
%A Fernandez-Muñoz, Beatriz
%A Barriga-Rivera, Alejandro
%A Navas-Garcia, Jose Manuel
%A Fernandez-Lizaranzu, Isabel
%A Munoz-Gonzalez, Francisco Javier
%A Parrilla-Giraldez, Ruben
%A Requena-Lancharro, Desiree
%A Guerrero-Claro, Manuel
%A Gil-Gamboa, Pedro
%A Rosell-Valle, Cristina
%A Gomez-Gonzalez, Carmen
%A Mayorga-Buiza, Maria Jose
%A Martin-Lopez, Maria
%A Muñoz, Olga
%A Martin, Juan Carlos Gomez
%A Lopez, Maria Isabel Relimpio
%A Aceituno-Castro, Jesus
%A Perales-Esteve, Manuel A
%A Puppo-Moreno, Antonio
%A Cozar, Francisco Jose Garcia
%A Olvera-Collantes, Lucia
%A de Los Santos-Trigo, Silvia
%A Gomez, Emilia
%A Pernaute, Rosario Sanchez
%A Padillo-Ruiz, Javier
%A Marquez-Rivas, Javier
%T Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples.
%D 2021
%U http://hdl.handle.net/10668/18356
%X Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU·[Formula: see text]L-1. This method constitutes an innovative approach that could be potentially used at point of care for rapid mass screening of viral infectious diseases and monitoring of the SARS-CoV-2 pandemic.
%K Red Andaluza de Diseño y Traslación de Terapias Avanzadas
%K Molecular diagnostic techniques
%K Point-of-care systems
%K Saliva
%K Sensitivity and specificity
%K Spectroscopy, near-infrared
%~