RT Journal Article T1 Biochemical Validation of a Self-Administered Food Frequency Questionnaire to Assess Diet Using Carotenoids and Vitamins E and D in Male Adolescents in Spain A1 Notario-Barandiaran, Leyre A1 Navarrete-Muñoz, Eva-María A1 Valera-Gran, Desirée A1 Hernández-Álvarez, Elena A1 Donoso-Navarro, Encarnación A1 González-Palacios, Sandra A1 García-de-la-Hera, Manuela A1 Fernández, Mariana F. A1 Freire, Carmen A1 Vioque, Jesús K1 Carotenoid intake K1 Vitamin E K1 Vitamin D K1 Fruit and vegetable intake K1 Food frequency questionnaire K1 Adolescence K1 Biochemical validity K1 Nutritional biomarker K1 Diet K1 Carotenoides K1 Vitamina E K1 Vitamina D K1 Frutas K1 Verduras K1 Ingestión de alimentos K1 Conducta alimentaria K1 Nutrición del adolescente K1 Dieta AB Reliable tools to evaluate diet are needed, particularly in life periods such as adolescence in which a rapid rate of growth and development occurs. We assessed the biochemical validity of a self-administered food frequency questionnaire (FFQ) in a sample of Spanish male adolescents using carotenoids and vitamin E and D data. We analyzed data from 122 male adolescents aged 15-17 years of the INMA-Granada birth cohort study. Adolescents answered a 104-item FFQ and provided a non-fasting blood sample. Mean daily nutrient intakes and serum concentration were estimated for main carotenoids (lutein-zeaxanthin, β-cryptoxanthin, lycopene, α-carotene and β-carotene), vitamins E and D and also for fruit and vegetable intake. Pearson correlation coefficients (r) and the percentage of agreement (same or adjacent quintiles) between serum vitamin concentrations and energy-adjusted intakes were estimated. Statistically significant correlation coefficients were observed for the total carotenoids (r = 0.40) and specific carotenoids, with the highest correlation observed for lutein-zeaxanthin (r = 0.42) and the lowest for β-carotene (0.23). The correlation coefficient between fruit and vegetable intake and serum carotenoids was 0.29 (higher for vegetable intake, r = 0.33 than for fruit intake, r = 0.19). Low correlations were observed for vitamin E and D. The average percentage of agreement for carotenoids was 55.8%, and lower for vitamin E and D (50% and 41%, respectively). The FFQ may be an acceptable tool for dietary assessment among male adolescents in Spain. PB MDPI YR 2021 FD 2021-05-08 LK http://hdl.handle.net/10668/4476 UL http://hdl.handle.net/10668/4476 LA en NO Notario-Barandiaran L, Navarrete-Muñoz EM, Valera-Gran D, Hernández-Álvarez E, Donoso-Navarro E, González-Palacios S, et al. Biochemical Validation of a Self-Administered Food Frequency Questionnaire to Assess Diet Using Carotenoids and Vitamins E and D in Male Adolescents in Spain. Antioxidants. 2021 May 8;10(5):750 DS RISalud RD Apr 7, 2025