RT Journal Article
T1 Neonatal Androgen Exposure Causes Persistent Gut Microbiota Dysbiosis Related to Metabolic Disease in Adult Female Rats
A1 Moreno-Indias, Isabel
A1 Sanchez-Alcoholado, Lidia
A1 Angel Sanchez-Garrido, Miguel
A1 Maria Martin-Nunez, Gracia
A1 Perez-Jimenez, Francisco
A1 Tena-Sempere, Manuel
A1 Tinahones, Francisco J.
A1 Isabel Queipo-Ortuno, Maria
K1 Insulin-resistance
K1 Energy-balance
K1 Body-fat
K1 Testosterone
K1 Obesity
K1 Bacteria
K1 Impact
K1 Diet
K1 Estradiol
K1 Estrogens
AB Alterations of gut microbiome have been proposed to play a role in metabolic disease, but the major determinants of microbiota composition remain ill defined. Nutritional and sex hormone challenges, especially during early development, have been shown to permanently alter adult female phenotype and contribute to metabolic disturbances. In this study, we implemented largescale microbiome analyses to fecal samples from groups of female rats sequentially subjected to various obesogenic manipulations, including sex hormone perturbations by means of neonatal and rogenization or adult ovariectomy (OVX), as a model of menopause, to establish whether these phenomena are related to changes in gut microbiota. Basic metabolic profiles concerning glucose/insulin homeostasis were also explored. The effects of the sex hormonal perturbations, either developmentally (androgenization) or in adulthood (OVX), clearly outshone the impact of nutritional interventions, especially concerning the gut microbiota profile. Notably, we observed a lower diversity in the androgenized group, with the highest Firmicutes to Bacteroidetes ratio, supporting the occurrence of durable alterations in gut microbiota composition, even in adulthood. Moreover, the elimination of adult ovarian secretions by OVX affected the richness of gut microbiota. Our data are the first to document the durable impact of sex steroid manipulations, and particularly early androgenization, on gut microbiota composition. Such dysbiosis is likely to contribute to the metabolic perturbations of conditions of obesity linked to gonadal dysfunction in the female.
PB Oxford univ press inc
SN 0013-7227
YR 2016
FD 2016-12-01
LK http://hdl.handle.net/10668/19138
UL http://hdl.handle.net/10668/19138
LA en
DS RISalud
RD Apr 11, 2025