RT Journal Article
T1 Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene.
A1 Thirion, Laurence
A1 Pezzi, Laura
A1 Pedrosa-Corral, Irene
A1 Sanbonmatsu-Gamez, Sara
A1 de Lamballerie, Xavier
A1 Falchi, Alessandra
A1 Perez-Ruiz, Mercedes
A1 Charrel, Remi N
K1 Mediterranean
K1 Phenuiviridae
K1 Phlebotomus
K1 Phlebovirus
K1 arbovirus
K1 diagnostics
K1 meningitis
K1 sand fly
AB Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
SN 2076-0817
YR 2021
FD 2021-02-24
LK https://hdl.handle.net/10668/28177
UL https://hdl.handle.net/10668/28177
LA en
DS RISalud
RD Apr 8, 2025