%0 Journal Article
%A Thirion, Laurence
%A Pezzi, Laura
%A Pedrosa-Corral, Irene
%A Sanbonmatsu-Gamez, Sara
%A de Lamballerie, Xavier
%A Falchi, Alessandra
%A Perez-Ruiz, Mercedes
%A Charrel, Remi N
%T Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene.
%D 2021
%@ 2076-0817
%U https://hdl.handle.net/10668/28177
%X Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
%K Mediterranean
%K Phenuiviridae
%K Phlebotomus
%K Phlebovirus
%K arbovirus
%K diagnostics
%K meningitis
%K sand fly
%~