RT Journal Article
T1 Osmanicin, a Polyketide Alkaloid Isolated from Streptomyces osmaniensis CA-244599 Inhibits Elastase in Human Fibroblasts.
A1 Samy, Mamdouh N
A1 Le Goff, Géraldine
A1 Lopes, Philippe
A1 Georgousaki, Katerina
A1 Gumeni, Sentiljana
A1 Almeida, Celso
A1 González, Ignacio
A1 Genilloud, Olga
A1 Trougakos, Ioannis
A1 Fokialakis, Nikolas
A1 Ouazzani, Jamal
K1 Streptomyces osmaniensis
K1 alkaloids
K1 elastase inhibition
K1 in situ solid-phase extraction
K1 osmanicin
AB The strain Streptomyces osmaniensis CA-244599 isolated from the Comoros islands was submitted to liquid-state fermentation coupled to in situ solid-phase extraction with amberlite XAD-16 resin. Elution of the trapped compounds on the resin beads by ethyl acetate afforded seven metabolites, osmanicin (1), streptazolin (2), streptazone C (3), streptazone B1 (4), streptenol C (5), nocardamine (6) and desmethylenylnocardamine (7). Osmanicin (1) is a newly reported unusual scaffold combining streptazolin (2) and streptazone C (3) through a Diels-Alder type reaction. Experimental evidence excluded the spontaneous formation of 1 from 2 and 3. The isolated compounds were evaluated for their ability to inhibit elastase using normal human diploid fibroblasts. Compound 1 exhibited the most potent activity with an IC50 of 3.7 μM.
YR 2019
FD 2019-06-15
LK http://hdl.handle.net/10668/14131
UL http://hdl.handle.net/10668/14131
LA en
DS RISalud
RD Apr 7, 2025