RT Journal Article
T1 High ACSL5 transcript levels associate with systemic lupus erythematosus and  apoptosis in Jurkat T lymphocytes and peripheral blood cells
A1 Catalá-Rabasa, Antonio
A1 Ndagire, Dorothy
A1 Sabio, Jose Mario
A1 Fedetz, María
A1 Matesanz, Fuencisla
A1 Alcina, Antonio
K1 Biología Celular
K1 Biología Computacional
K1 Genómica
K1 Genética
K1 Alergia e Inmunología
K1 Biología Molecular
K1 Reumatología
K1 Cell Biology
K1 Computational Biology
K1 Genetics and Genomics
K1 Immunology
K1 Molecular Biology
K1 Rheumatology
AB BACKGROUND: Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease in which increased apoptosis and decreased apoptotic cells removal has been described as most relevant in the pathogenesis. Long-chain acyl-coenzyme A synthetases (ACSLs) have been involved in the immunological dysfunction of mouse models of lupus-like autoimmunity and apoptosis in different in vitro cell systems. The aim of this work was to assess among the ACSL isoforms the  involvement of ACSL2, ACSL4 and ACSL5 in SLE pathogenesis. FINDINGS: With this end, we determined the ACSL2, ACSL4 and ACSL5 transcript levels in peripheral blood mononuclear cells (PBMCs) of 45 SLE patients and 49 healthy controls by quantitative real time-PCR (q-PCR). We found that patients with SLE had higher ACSL5 transcript levels than healthy controls [median (range), healthy controls =16.5 (12.3-18.0) vs. SLE = 26.5 (17.8-41.7), P = 3.9x10 E-5] but no differences were found for ACSL2 and ACSL4. In in vitro experiments, ACSL5 mRNA expression was greatly increased when inducing apoptosis in Jurkat T cells and PBMCs by Phorbol-Myristate-Acetate plus Ionomycin (PMA+Io). On the other hand, shortinterference RNA (siRNA)-mediated silencing of ACSL5 decreased induced apoptosis in Jurkat T cells up to the control levels as well as decreased mRNA expression of FAS, FASLG and TNF. CONCLUSIONS: These findings indicate that ACSL5 may play a role in the apoptosis that takes place in SLE. Our results point to ACSL5 as a potential novel functional marker of pathogenesis and a possible therapeutic target in SLE
PB Public Library of Science
YR 2011
FD 2011-12-06
LK http://hdl.handle.net/10668/410
UL http://hdl.handle.net/10668/410
LA en
NO Catalá-Rabasa A, Ndagire D, Sabio JM, Fedetz M, Matesanz F, Alcina A.  High ACSL5 Transcript Levels Associate with Systemic Lupus Erythematosus and Apoptosis in Jurkat T Lymphocytes and Peripheral Blood Cells. PLoS ONE .2011 ; 6(12): e28591.
DS RISalud
RD Apr 4, 2025