RT Journal Article
T1 Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos.
A1 Carmona, Rita
A1 Cano, Elena
A1 Mattiotti, Andrea
A1 Gaztambide, Joaquín
A1 Muñoz-Chápuli, Ramón
K1 Diferenciación Celular
K1 Linaje celular
K1 Canales del cloro
K1 Células epiteliales
K1 Ratones transgénicos
K1 Factor 9 de crecimiento de fibroblastos
K1 Intestinos
K1 Músculo liso
K1 Receptor alfa de factor de crecimiento derivados de plaquetas
AB Gut mesodermal tissues originate from the splanchnopleural mesenchyme. However, the embryonic gastrointestinal coelomic epithelium gives rise to mesenchymal cells, whose significance and fate are little known. Our aim was to investigate the contribution of coelomic epithelium-derived cells to the intestinal development. We have used the transgenic mouse model mWt1/IRES/GFP-Cre (Wt1(cre)) crossed with the Rosa26R-EYFP reporter mouse. In the gastrointestinal duct Wt1, the Wilms' tumor suppressor gene, is specific and dynamically expressed in the coelomic epithelium. In the embryos obtained from the crossbreeding, the Wt1-expressing cell lineage produces the yellow fluorescent protein (YFP) allowing for colocalization with differentiation markers through confocal microscopy and flow cytometry. Wt1(cre-YFP) cells were very abundant throughout the intestine during midgestation, declining in neonates. Wt1(cre-YFP) cells were also transiently observed within the mucosa, being apparently released into the intestinal lumen. YFP was detected in cells contributing to intestinal vascularization (endothelium, pericytes and smooth muscle), visceral musculature (circular, longitudinal and submucosal) as well as in Cajal and Cajal-like interstitial cells. Wt1(cre-YFP) mesenchymal cells expressed FGF9, a critical growth factor for intestinal development, as well as PDGFRα, mainly within developing villi. Thus, a cell population derived from the coelomic epithelium incorporates to the gut mesenchyme and contribute to a variety of intestinal tissues, probably playing also a signaling role. Our results support the origin of interstitial cells of Cajal and visceral circular muscle from a common progenitor expressing anoctamin-1 and SMCα-actin. Coelomic-derived cells contribute to the differentiation of at least a part of the interstitial cells of Cajal.
PB Public Library of Science
YR 2013
FD 2013-02-13
LK http://hdl.handle.net/10668/1393
UL http://hdl.handle.net/10668/1393
LA en
NO Carmona R, Cano E, Mattiotti A, Gaztambide J, Muñoz-Chápuli R. Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos. PLoS ONE. 2013; 8(2):e55890
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 11, 2025