RT Journal Article
T1 Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves
A1 García-García, Óscar Darío
A1 El Soury, Marwa
A1 Gonzalez-Quevedo, David
A1 Sánchez-Porras, David
A1 Chato-Astrain, Jesús
A1 Campos, Fernando
A1 Carriel, Víctor
K1 TIssue engineering
K1 Nerve repair
K1 Nerve tissue decellularization
K1 Genipin
K1 Chemical crosslinking
K1 Histology
K1 Natural biomaterials
K1 Biomechanical and structural properties
K1 Cellbiomaterials interactions
K1 Ingeniería de tejidos
K1 Histología
K1 Andamios del tejido
K1 Regeneración nerviosa
K1 Nervio ciático
K1 Fenómenos biomecánicos
AB Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.
PB MDPI
YR 2021
FD 2021-01-12
LK http://hdl.handle.net/10668/3516
UL http://hdl.handle.net/10668/3516
LA en
NO García-García ÓD, El Soury M, González-Quevedo D, Sánchez-Porras D, Chato-Astrain J, Campos F, et al. Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves. International Journal of Molecular Sciences. 2021 Jan 12;22(2):674
DS RISalud
RD Apr 9, 2025