RT Journal Article
T1 Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice.
A1 Leyva-Jiménez, Francisco Javier
A1 Ruiz-Malagón, Antonio Jesús
A1 Molina-Tijeras, José Alberto
A1 Diez-Echave, Patricia
A1 Vezza, Teresa
A1 Hidalgo-García, Laura
A1 Lozano-Sánchez, Jesús
A1 Arráez-Román, David
A1 Cenis, José Luis
A1 Lozano-Pérez, Antonio Abel
A1 Rodríguez-Nogales, Alba
A1 Segura-Carretero, Antonio
A1 Gálvez, Julio
K1 Morus alba leaves
K1 anti-inflammatory
K1 chemical characterization
K1 high fat diet
K1 metabolic disorders
K1 obesity
AB Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography-electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH· radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.
SN 2076-3921
YR 2020
FD 2020-08-11
LK https://hdl.handle.net/10668/26804
UL https://hdl.handle.net/10668/26804
LA en
DS RISalud
RD Apr 8, 2025