RT Journal Article
T1 IL8 and IL16 levels indicate serum and plasma quality.
A1 Kofanova, Olga
A1 Henry, Estelle
A1 Aguilar Quesada, Rocio
A1 Bulla, Alexandre
A1 Navarro Linares, Hector
A1 Lescuyer, Pierre
A1 Shea, Kathi
A1 Stone, Mars
A1 Tybring, Gunnel
A1 Bellora, Camille
A1 Betsou, Fay
K1 interleukin
K1 plasma
K1 preanalytical
K1 quality control
K1 serum
AB Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified. Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their "diagnostic performance" in identifying serum or plasma samples with extended pre-centrifugation times. In this study, using R&D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL. These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.
YR 2018
FD 2018
LK http://hdl.handle.net/10668/12108
UL http://hdl.handle.net/10668/12108
LA en
DS RISalud
RD Apr 8, 2025