%0 Journal Article
%A Ceperuelo-Mallafre, Victoria
%A Reverte, Laia
%A Peraire, Joaquim
%A Madeira, Ana
%A Maymo-Masip, Elsa
%A Lopez-Dupla, Miguel
%A Gutierrez-Valencia, Alicia
%A Ruiz-Mateos, Ezequiel
%A Buzon, Maria Jose
%A Jorba, Rosa
%A Vendrell, Joan
%A Auguet, Teresa
%A Olona, Montserrat
%A Vidal, Francesc
%A Rull, Anna
%A Fernandez-Veledo, Sonia
%T Circulating pyruvate is a potent prognostic marker for critical COVID-19 outcomes.
%D 2022
%U http://hdl.handle.net/10668/20589
%X Coronavirus-19 (COVID-19) disease is driven by an unchecked immune response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus which alters host mitochondrial-associated mechanisms. Compromised mitochondrial health results in abnormal reprogramming of glucose metabolism, which can disrupt extracellular signalling. We hypothesized that examining mitochondrial energy-related signalling metabolites implicated in host immune response to SARS-CoV-2 infection would provide potential biomarkers for predicting the risk of severe COVID-19 illness. We used a semi-targeted serum metabolomics approach in 273 patients with different severity grades of COVID-19 recruited at the acute phase of the infection to determine the relative abundance of tricarboxylic acid (Krebs) cycle-related metabolites with known extracellular signaling properties (pyruvate, lactate, succinate and α-ketoglutarate). Abundance levels of energy-related metabolites were evaluated in a validation cohort (n=398) using quantitative fluorimetric assays. Increased levels of four energy-related metabolites (pyruvate, lactate, a-ketoglutarate and succinate) were found in critically ill COVID-19 patients using semi-targeted and targeted approaches (p Our findings support the link between COVID-19 pathogenesis and immunometabolic dysregulation, and show that fluorometric quantification of circulating pyruvate is a cost-effective clinical decision support tool to improve patient stratification and prognosis prediction.
%K COVID-19
%K energy-related metabolites
%K fluorometric quantification
%K pyruvate
%K semi-targeted metebolomics
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