RT Journal Article
T1 Synthesis and Characterization of Elongated-Shaped Silver Nanoparticles as a Biocompatible Anisotropic SERS Probe for Intracellular Imaging: Theoretical Modeling and Experimental Verification.
A1 Caro, Carlos
A1 Quaresma, Pedro
A1 Pereira, Eulália
A1 Franco, Jaime
A1 Pernia Leal, Manuel
A1 García-Martín, Maria Luisa
A1 Royo, Jose Luis
A1 Oliva-Montero, Jose Maria
A1 Merkling, Patrick Jacques
A1 Zaderenko, Ana Paula
A1 Pozo, David
A1 Franco, Ricardo
K1 SERS
K1 cancer
K1 cell labeling
K1 density functional theory calculations
K1 finite element method
K1 surface enhanced Raman scattering
AB Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 10⁷) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe.
SN 2079-4991
YR 2019
FD 2019-02-13
LK http://hdl.handle.net/10668/13590
UL http://hdl.handle.net/10668/13590
LA en
DS RISalud
RD Apr 7, 2025