RT Journal Article
T1 Lack of cytomegalovirus (CMV)-specific cell-mediated immune response using QuantiFERON-CMV assay in CMV-seropositive healthy volunteers: fact not artifact.
A1 Valle-Arroyo, Jorge
A1 Aguado, Rocio
A1 Paez-Vega, Aurora
A1 Perez, Ana B
A1 Gonzalez, Rafael
A1 Fornes, Gema
A1 Torre-Cisneros, Julian
A1 Cantisan, Sara
K1 Adult
K1 Antibodies, viral
K1 CD4-positive T-lymphocytes
K1 CD8-positive T-lymphocytes
K1 Cytomegalovirus
AB The QuantiFERON-CMV (QF) assay measures cell-mediated immunity against cytomegalovirus (CMV-CMI), which is particularly useful in individuals susceptible to CMV infection such as transplant patients. A positive QF result identifies patients that are better protected against CMV infection. However, the significance of a negative QF result in CMV-seropositive individuals needs to be clarified. CMV-CMI was analyzed in healthy subjects using the QF assay, and, in parallel, the Flow-cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood (FASCIA). FASCIA assay measures T-cell proliferation using CMV lysate as stimulus whereas QF assay use a mix of peptides. A total of 93 healthy volunteers were enrolled, and 13/71 CMV-seropositive individuals (18.3%) showed humoral/cellular discordance using QF assay (CMV+ QF-). Interestingly, with FASCIA assay CD4+ and CD8+ T-cell proliferations were lower in CMV+ QF- than in CMV+ QF+ individuals. Furthermore, CMV+ QF- volunteers had a lower level of anti-CMV IgG than CMV+ QF+ subjects. Discordant CMV+ QF- volunteers can be defined as low responder individuals since they show lower CMV-specific humoral and cellular immune responses in comparison to CMV+ QF+ individuals. Immune discordance shows the high heterogeneity of immunity to CMV in healthy subjects.
PB Nature Publishing Group
YR 2020
FD 2020-04-08
LK http://hdl.handle.net/10668/15457
UL http://hdl.handle.net/10668/15457
LA en
NO Valle-Arroyo J, Aguado R, Páez-Vega A, Pérez AB, González R, Fornés G, et al. Lack of cytomegalovirus (CMV)-specific cell-mediated immune response using QuantiFERON-CMV assay in CMV-seropositive healthy volunteers: fact not artifact. Sci Rep. 2020 Apr 28;10(1):7194
NO Tis work was supported by National R&D&I Plans 2008–2011/2013–2016 and the Instituto de Salud Carlos III, Sub-Directorate General for Cooperative Research Networks and Centers, Spanish Ministry of Economy and Competitiveness, Spanish Network for Research in Infectious Diseases (grant number REIPI RD16/0016/0008).
DS RISalud
RD Apr 6, 2025