RT Journal Article
T1 Prognostic Role of the Expression of Latent-Membrane Protein 1 of Epstein-Barr Virus in Classical Hodgkin Lymphoma
A1 Santisteban-Espejo, Antonio
A1 Perez-Requena, Jose
A1 Atienza-Cuevas, Lidia
A1 Moran-Sanchez, Julia
A1 del Carmen Fernandez-Valle, Maria
A1 Bernal-Florindo, Irene
A1 Romero-Garcia, Raquel
A1 Garcia-Rojo, Marcial
K1 B-cell lymphomas
K1 classical Hodgkin lymphoma
K1 Epstein-Barr virus
K1 Latent-Membrane Protein 1
K1 risk-adjusted therapy
K1 Age-defined subgroups
K1 Reed-sternberg cells
K1 B-cells
K1 Presenting features
K1 Disease
K1 Impact
K1 Ebv
K1 Infection
K1 Lmp1
K1 Membrane-protein-1
AB The prognostic impact of the presence of Epstein-Barr virus (EBV) in classical Hodgkin lymphoma (cHL) is controversial. Previous studies reported heterogeneous results, rendering difficult the clinical validation of EBV as a prognostic biomarker in this lymphoma. The objective of this study was to evaluate the survival impact of the expression of EBV Latent-Membrane Protein 1 (EBV-LMP1) in tumoral Hodgkin-Reed-Sternberg (HRS) cells of primary diagnostic samples of cHL. Formalin-Fixed Paraffin-Embedded (FFPE) lymph node samples from 88 patients with cHL were analyzed. Patients were treated with the standard first-line chemotherapy (CT) with Adriamycin, Bleomycin, Vinblastine and Dacarbazine (ABVD) followed by radiotherapy. The Kaplan-Meier method and the Cox proportional hazards model were used for carrying out the survival analysis. In order to investigate whether the influence of EBV was age-dependent, analyses were performed both for patients of all ages and for age-stratified subgroups. In bivariate analysis, the expression of EBV was associated with older age (p = 0.011), mixed cellularity subtype cHL (p < 0.001) and high risk International Prognostic Score (IPS) (p = 0.023). Overall survival (OS) and progression-free survival (PFS) were associated with the presence of bulky disease (p = 0.009) and advanced disease at diagnosis (p = 0.016). EBV-positive cases did not present a significantly lower OS and PFS in comparison with EBV-negative cases, for all ages and when stratifying for age. When adjusted for covariates, absence of bulky disease at diagnosis (HR: 0.102, 95% CI: 0.02-0.48, p = 0.004) and limited disease stages (I-II) (HR: 0.074, 95% CI: 0.01-0.47, p = 0.006) were associated with a significant better OS. For PFS, limited-disease stages also retained prognostic impact in the multivariate Cox regression (HR: 0.145, 95% CI: 0.04-0.57, p = 0.006). These results are of importance as the early identification of prognostic biomarkers in cHL is critical for guiding and personalizing therapeutic decisions. The prognostic role of EBV in cHL could be modulated by the type of CT protocol employed and interact with the rest of presenting features.
PB Mdpi
SN 1999-4915
YR 2021
FD 2021-12-15
LK https://hdl.handle.net/10668/25268
UL https://hdl.handle.net/10668/25268
LA en
NO Santisteban-Espejo A, Perez-Requena J, Atienza-Cuevas L, Moran-Sanchez J, del Carmen Fernandez-Valle M, Bernal-Florindo I, et al. Prognostic Role of the Expression of Latent-Membrane Protein 1 of Epstein-Barr Virus in Classical Hodgkin Lymphoma. Viruses. 2021;13(12):2523. Published 2021 Dec 15.
NO This work has been supported by a postdoctoral grant (RH-0145-2020) from the Andalusia Health System and with the EU FEDER ITI Grant for Cadiz Province PI-0032-2017.
DS RISalud
RD Sep 27, 2025