RT Journal Article T1 Characterization of the secretory profile and exosomes of limbal stem cells in the canine species A1 Villatoro, Antonio J. A1 Alcoholado, Cristina A1 Martín-Astorga, María del Carmen A1 Rico, Gustavo A1 Fernández, Viviana A1 Becerra, José K1 Dogs K1 Stem cells K1 Epithelium, corneal K1 Fibroblasts K1 Exosomes K1 Corneal diseases K1 Perros K1 Células madre K1 Epitelio corneal K1 Fibroblastos K1 Exosomas K1 Enfermedades de la cornea AB Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of the corneal epithelium. Their deficiency is responsible for the conjunctivization of the cornea that is seen in different ocular pathologies, both in humans and in the canine species. The canine species represents an interesting preclinical animal model in ocular surface pathologies. However, the role of LSCs in physiological and pathological conditions in canine species is not well understood. Our objective was to characterize for the first time the soluble factors and the proteomic profile of the secretome and exosomes of canine LSCs (cLSCs). In addition, given the important role that fibroblasts play in the repair of the ocular surface, we evaluated the influence of the secretome and exosomes of cLSCs on their proliferation in vitro. Our results demonstrated a secretory profile of cLSCs with high concentrations of MCP-1, IL-8, VEGF-A, and IL-10, as well as significant production of exosomes. Regarding the proteomic profile, 646 total proteins in the secretome and 356 in exosomes were involved in different biological processes. Functionally, the cLSC secretome showed an inhibitory effect on the proliferation of fibroblasts in vitro, which the exosomes did not. These results open the door to new studies on the possible use of the cLSC secretome or some of its components to treat certain pathologies of the ocular surface in canine species. PB Alexander V. Ljubimov, Cedars-Sinai Medical Center, United States. Editor comercial: Public Library of Science. PLOS YR 2020 FD 2020-12-29 LK http://hdl.handle.net/10668/3504 UL http://hdl.handle.net/10668/3504 LA en NO Villatoro AJ, Alcoholado C, Martín-Astorga MC, Rico G, Fernández V, Becerra J. Characterization of the secretory profile and exosomes of limbal stem cells in the canine species. PLoS One. 2020 Dec 29;15(12):e0244327. DS RISalud RD Apr 5, 2025