RT Journal Article
T1 Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles
A1 Vurro, Federica
A1 Jabalera, Ylenia
A1 Mannucci, Silvia
A1 Glorani, Giulia
A1 Sola-Leyva, Alberto
A1 Gerosa, Marco
A1 Romeo, Alessandro
A1 Romanelli, Maria Grazia
A1 Malatesta, Manuela
A1 Calderan, Laura
A1 Iglesias, Guillermo R.
A1 Carrasco-Jiménez, María P.
A1 Jimenez-Lopez, Concepcion
A1 Perduca, Massimiliano
K1 Biomimetic magnetic nanoparticles
K1 Poly (lactic-co-glycolic) acid
K1 PLGA
K1 Penetrating TAT peptide
K1 Nanoparticles
K1 Magnetic hyperthermia
K1 Cellular uptake
K1 Magnetic fields
K1 Nanopartículas magnéticas de óxido de hierro
K1 Copolímero de ácido poliláctico-ácido poliglicólico
K1 Nanopartículas
K1 Hipertermia
K1 Campos magnéticos
AB Magnetococcus marinus magnetosome-associated protein MamC, expressed as recombinant, has been proven to mediate the formation of novel biomimetic magnetic nanoparticles (BMNPs) that are successful drug nanocarriers for targeted chemotherapy and hyperthermia agents. These BMNPs present several advantages over inorganic magnetic nanoparticles, such as larger sizes that allow the former to have larger magnetic moment per particle, and an isoelectric point at acidic pH values, which allows both the stable functionalization of BMNPs at physiological pH value and the molecule release at acidic (tumor) environments, simply based on electrostatic interactions. However, difficulties for BMNPs cell internalization still hold back the efficiency of these nanoparticles as drug nanocarriers and hyperthermia agents. In the present study we explore the enhanced BMNPs internalization following upon their encapsulation by poly (lactic-co-glycolic) acid (PLGA), a Food and Drug Administration (FDA) approved molecule. Internalization is further optimized by the functionalization of the nanoformulation with the cell-penetrating TAT peptide (TATp). Our results evidence that cells treated with the nanoformulation [TAT-PLGA(BMNPs)] show up to 80% more iron internalized (after 72 h) compared to that of cells treated with BMNPs (40%), without any significant decrease in cell viability. This nanoformulation showing optimal internalization is further characterized. In particular, the present manuscript demonstrates that neither its magnetic properties nor its performance as a hyperthermia agent are significantly altered due to the encapsulation. In vitro experiments demonstrate that, following upon the application of an alternating magnetic field on U87MG cells treated with BMNPs and TAT-PLGA(BMNPs), the cytotoxic effect of BMNPs was not affected by the TAT-PLGA enveloping. Based on that, difficulties shown in previous studies related to poor cell uptake of BMNPs can be overcome by the novel nanoassembly described here.
PB MDPI
YR 2021
FD 2021-03-18
LK http://hdl.handle.net/10668/4463
UL http://hdl.handle.net/10668/4463
LA en
NO Vurro F, Jabalera Y, Mannucci S, Glorani G, Sola-Leyva A, Gerosa M, et al. Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles. Nanomaterials. 2021 Mar 18;11(3):766
DS RISalud
RD Apr 7, 2025