RT Journal Article
T1 Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats.
A1 Ferreyra, Carla
A1 Vargas, Félix
A1 Rodríguez-Gómez, Isabel
A1 Pérez-Abud, Rocío
A1 O'Valle, Francisco
A1 Osuna, Antonio
K1 Animales
K1 Ratas
K1 Triyodotironina
K1 Necrosis Tubular Aguda
K1 Isquemia
K1 Reperfusión
K1 Lesión Renal Aguda
K1 Estrés Oxidativo
K1 Preacondicionamiento Isquémico
AB BACKGROUNDRenal ischemia/reperfusion (I/R) injury is manifested by acute renal failure (ARF) and acute tubular necrosis (ATN). The aim of this study was to evaluate the effectiveness of preconditioning with 3, 3, 5 triiodothyronine (T3) to prevent I/R renal injury.METHODOLOGY/PRINCIPAL FINDINGSTHE RATS WERE DIVIDED INTO FOUR GROUPS: sham-operated, placebo-treated (SO-P), sham-operated T3- treated (SO- T3), I/R-injured placebo-treated (IR-P), and I/R-injured T3-treated (IR- T3) groups. At 24 h before ischemia, the animals received a single dose of T3 (100 μg/kg). Renal function and plasma, urinary, and tissue variables were studied at 4, 24, and 48 h of reperfusion, including biochemical, oxidative stress, and inflammation variables, PARP-1 immunohistochemical expression, and ATN morphology. In comparison to the SO groups, the IR-P groups had higher plasma urea and creatinine levels and greater proteinuria (at all reperfusion times) and also showed: increased oxidative stress-related plasma, urinary, and tissue variables; higher plasma levels of IL6 (proinflammatory cytokine); increased glomerular and tubular nuclear PARP-1 expression; and a greater degree of ATN. The IR-T3 group showed a marked reduction in all of these variables, especially at 48 h of reperfusion. No significant differences were observed between SO-P and SO-T3 groups.CONCLUSIONSThis study demonstrates that preconditioning rats with a single dose of T3 improves the clinical signs and ATN of renal I/R injury. These beneficial effects are accompanied by reductions in oxidative stress, inflammation, and renal PARP-1 expression, indicating that this sequence of factors plays an important role in the ATN induced by I/R injury.
PB Public Library of Science
YR 2013
FD 2013-09-26
LK http://hdl.handle.net/10668/1318
UL http://hdl.handle.net/10668/1318
LA en
NO Ferreyra C, Vargas F, Rodríguez-Gómez I, Pérez-Abud R, O'Valle F, Osuna A. Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats. PLoS ONE. 2013; 8(9):e74960
NO JOURNAL ARTICLE;
DS RISalud
RD Apr 13, 2025