RT Journal Article
T1 Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning.
A1 Mayoral, Isabel
A1 Bevilacqua, Elisa
A1 Gómez, Gorka
A1 Hmadcha, Abdelkrim
A1 González-Loscertales, Ignacio
A1 Reina, Esther
A1 Sotelo, Julio
A1 Domínguez, Antonia
A1 Pérez-Alcántara, Pedro
A1 Smani, Younes
A1 González-Puertas, Patricia
A1 Mendez, Ana
A1 Uribe, Sergio
A1 Smani, Tarik
A1 Ordoñez, Antonio
A1 Valverde, Israel
K1 3D printing
K1 Electrospinning
K1 Endothelin Receptor A, ETA
K1 Endothelin Receptor B, ETB
K1 Mesenchymal stem cells
K1 Reverse Transcription, Rt
K1 Three-dimensional, 3D
K1 Tissue engineering
K1 Vascular graft
K1 anti-alpha-smooth muscle actin, α-SMA
K1 anti-cluster of differentiation 31, CD31
K1 anti-fibroblast specific protein 1, FSP1
K1 anti-smooth muscle protein 22, SM-22
K1 bone morphogenetic protein, BMP4
K1 computation fluid dynamic, CFD
K1 computed tomography, CT
K1 derived VSMC, dVSMC
K1 endothelin-1, ET-1
K1 extracellular matrix, ECM
K1 fused deposition modelling, FDM
K1 mesenchymal stem cells, MSC
K1 platelet-derived growth factor composed by two beta chains, PDGF-BB
K1 room temperature, RT
K1 tissue engineering vascular grafts, TEVG
K1 transforming growth factor beta 1, TGFβ-1
K1 vascular smooth muscle cells, VSMC
K1 wall shear stress, WSS
K1 western blotting, WB
AB Three-dimensional (3D) engineered cardiovascular tissues have shown great promise to replace damaged structures. Specifically, tissue engineering vascular grafts (TEVG) have the potential to replace biological and synthetic grafts. We aimed to design an in-vitro patient-specific patch based on a hybrid 3D print combined with vascular smooth muscle cells (VSMC) differentiation. Based on the medical images of a 2 months-old girl with aortic arch hypoplasia and using computational modelling, we evaluated the most hemodynamically efficient aortic patch surgical repair. Using the designed 3D patch geometry, the scaffold was printed using a hybrid fused deposition modelling (FDM) and electrospinning techniques. The scaffold was seeded with multipotent mesenchymal stem cells (MSC) for later maturation to derived VSMC (dVSMC). The graft showed adequate resistance to physiological aortic pressure (burst pressure 101 ​± ​15 ​mmHg) and a porosity gradient ranging from 80 to 10 ​μm allowing cells to infiltrate through the entire thickness of the patch. The bio-scaffolds showed good cell viability at days 4 and 12 and adequate functional vasoactive response to endothelin-1. In summary, we have shown that our method of generating patient-specific patch shows adequate hemodynamic profile, mechanical properties, dVSMC infiltration, viability and functionality. This innovative 3D biotechnology has the potential for broad application in regenerative medicine and potentially in heart disease prevention.
YR 2022
FD 2022-04-14
LK http://hdl.handle.net/10668/22400
UL http://hdl.handle.net/10668/22400
LA en
DS RISalud
RD Apr 6, 2025