RT Journal Article
T1 Native Chilean Berries Preservation and In Vitro Studies of a Polyphenol Highly Antioxidant Extract from Maqui as a Potential Agent against Inflammatory Diseases.
A1 Ortiz, Tamara
A1 Argüelles-Arias, Federico
A1 Begines, Belén
A1 García-Montes, Josefa-María
A1 Pereira, Alejandra
A1 Victoriano, Montserrat
A1 Vázquez-Román, Victoria
A1 Pérez Bernal, Juan Luis
A1 Callejón, Raquel M
A1 De-Miguel, Manuel
A1 Alcudia, Ana
K1 HT-29 cells
K1 RAW 264.7 cells
K1 antioxidant activity
K1 inflammation
K1 inflammatory bowel disease
K1 maqui berry extract
K1 oxidative stress
K1 polyphenols and anthocyanins content
K1 preservation methods
AB The best conservation method for native Chilean berries has been investigated in combination with an implemented large-scale extract of maqui berry, rich in total polyphenols and anthocyanin to be tested in intestinal epithelial and immune cells. The methanolic extract was obtained from lyophilized and analyzed maqui berries using Folin-Ciocalteu to quantify the total polyphenol content, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) to measure the antioxidant capacity. Determination of maqui's anthocyanins profile was performed by ultra-high-performance liquid chromatography (UHPLC-MS/MS). Viability, cytotoxicity, and percent oxidation in epithelial colon cells (HT-29) and macrophages cells (RAW 264.7) were evaluated. In conclusion, preservation studies confirmed that the maqui properties and composition in fresh or frozen conditions are preserved and a more efficient and convenient extraction methodology was achieved. In vitro studies of epithelial cells have shown that this extract has a powerful antioxidant strength exhibiting a dose-dependent behavior. When lipopolysaccharide (LPS)-macrophages were activated, noncytotoxic effects were observed, and a relationship between oxidative stress and inflammation response was demonstrated. The maqui extract along with 5-aminosalicylic acid (5-ASA) have a synergistic effect. All of the compiled data pointed out to the use of this extract as a potential nutraceutical agent with physiological benefits for the treatment of inflammatory bowel disease (IBD).
SN 2076-3921
YR 2021
FD 2021-05-25
LK http://hdl.handle.net/10668/17916
UL http://hdl.handle.net/10668/17916
LA en
DS RISalud
RD Apr 6, 2025