RT Journal Article
T1 Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients.
A1 Ortega, Francisco G
A1 Regiart, Matías D
A1 Rodríguez-Martínez, Alba
A1 de Miguel-Pérez, Diego
A1 Serrano, María J
A1 Lorente, José A
A1 Tortella, Gonzalo
A1 Rubilar, Olga
A1 Sapag, Karim
A1 Bertotti, Mauro
A1 Fernández-Baldo, Martín A
AB This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
YR 2020
FD 2020-12-10
LK https://hdl.handle.net/10668/28334
UL https://hdl.handle.net/10668/28334
LA en
DS RISalud
RD Apr 6, 2025