RT Journal Article
T1 Evaluation of lung parenchyma, blood vessels, and peripheral blood lymphocytes as a potential source of acute phase reactants in patients with COPD.
A1 Arellano-Orden, Elena
A1 Calero, Carmen
A1 López-Ramírez, Cecilia
A1 Sánchez-López, Verónica
A1 López-Villalobos, José Luis
A1 Abad Arranz, María
A1 Blanco-Orozco, Ana
A1 Otero-Candelera, Remedios
A1 López-Campos, José Luis
K1 C-reactive protein
K1 COPD
K1 arterial wall
K1 inflammation
K1 parenchyma
K1 serum amyloid A
AB Background: Previous studies have shown that the arterial wall is a potential source of inflammatory markers in COPD. Here, we sought to compare the expression of acute phase reactants (APRs) in COPD patients and controls both at the local (pulmonary arteries and lung parenchyma) and systemic (peripheral blood leukocytes and plasma) compartments. Methods: Consecutive patients undergoing elective surgery for suspected primary lung cancer were eligible for the study. Patients were categorized either as COPD or control group based on the spirometry results. Pulmonary arteries and lung parenchyma sections, peripheral blood leukocytes, and plasma samples were obtained from all participants. Gene expression levels of C-reactive protein (CRP) and serum amyloid A (SAA1, SAA2, and SAA4) were evaluated in tissue samples and peripheral blood leukocytes by reverse transciption-PCR. Plasma CRP and SAA protein levels were measured by enzyme-linked immunosorbent assays. Proteins were evaluated in paraffin-embedded lung tissues by immunohistochemistry. Results: A total of 40 patients with COPD and 62 controls were enrolled. We did not find significant differences in the gene expression between COPD and control group. Both CRP and SAA were overexpressed in the lung parenchyma compared with pulmonary arteries and peripheral blood leukocytes. The expression of SAA was significantly higher in the lung parenchyma than in the pulmonary artery (2-fold higher for SAA1 and SAA4, P=0.015 and P
YR 2019
FD 2019-06-20
LK http://hdl.handle.net/10668/14399
UL http://hdl.handle.net/10668/14399
LA en
DS RISalud
RD Apr 10, 2025