RT Journal Article
T1 Promoter Genotyping and mRNA Expression - Based Analysis of the PTGDR Gene in Allergy.
A1 San Segundo-Val, I
A1 García-Sánchez, A
A1 Sanz, C
A1 Cornejo-García, J A
A1 Isidoro-García, M
A1 Dávila, I
K1 Allergy
K1 Asthma
K1 PTGDR
K1 Polymorphisms
K1 Rhinitis
K1 mRNA expression
AB Prostaglandin D2 receptors are acquiring a relevant role as potential therapeutic targets in allergy. PTGDR has been described as a candidate gene in allergic disease, although functional studies on this gene are lacking. Objective: The objective of this case-control study was to investigate the potential role of PTGDR in allergy. The study population comprised 195 allergic patients and 112 healthy controls. The PTGDR promoter polymorphisms -1289G>A, -1122T>C, -881C>T, -834C>T, -613C>T, -549T>C, -441C>T, -197T>C, and -95G>T were amplified by polymerase chain reaction (PCR) and sequenced. PTGDR expression levels were analyzed using quantitative PCR and normalized to GAPDH and TBP mRNA levels. All procedures were performed following the Minimum Information for Publication of Quantitative Real-Time PCR Experiment guidelines. PTGDR expression levels were significantly higher in allergic patients than in controls (PA and -1122T>C were found in allergic patients (P=.009). The results indicate that PTGDR overexpression is associated with allergy. The polymorphisms -1289G>A and -1122T>C partly explain the variation in expression we observed. PTGDR expression could have a potential role as a biomarker and pharmacogenetic factor in allergy.
SN 1018-9068
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10668/13920
UL http://hdl.handle.net/10668/13920
LA en
DS RISalud
RD Apr 11, 2025