RT Journal Article
T1 Phylodynamic and Phylogeographic Profiles of Subtype B HIV-1 Epidemics in South Spain.
A1 Pérez-Parra, Santiago
A1 Chueca, Natalia
A1 Álvarez, Marta
A1 Pasquau, Juan
A1 Omar, Mohamed
A1 Collado, Antonio
A1 Vinuesa, David
A1 Lozano, Ana B
A1 Yebra, Gonzalo
A1 García, Federico
K1 Linfocitos T CD4-positivos
K1 Ciudades
K1 Análisis por conglomerados
K1 Epidemias
K1 Genotipo
K1 Infecciones por VIH
K1 VIH-1
K1 Homosexualidad masculina
K1 Péptido hidrolasas
K1 Filogeografía
K1 Probabilidad
K1 ADN polimerasa dirigida por ARN
K1 España
K1 Teorema de bayes
AB BACKGROUNDSince 1982, HIV-1 epidemics have evolved to different scenarios in terms of transmission routes, subtype distribution and characteristics of transmission clusters. We investigated the evolutionary history of HIV-1 subtype B in south Spain.PATIENTS & METHODSWe studied all newly diagnosed HIV-1 subtype B patients in East Andalusia during the 2005-2012 period. For the analysis, we used the reverse transcriptase and protease sequences from baseline resistance, and the Trugene® HIV Genotyping kit (Siemens, Barcelona, Spain). Subtyping was done with REGA v3.0. The maximum likelihood trees constructed with RAxML were used to study HIV-1 clustering. Phylogeographic and phylodynamic profiles were studied by Bayesian inference methods with BEAST v1.7.5 and SPREAD v1.0.6.RESULTSOf the 493 patients infected with HIV-1 subtype B, 234 grouped into 55 clusters, most of which were small (44 clusters ≤ 5 patients, 31 with 2 patients, 13 with 3). The rest (133/234) were grouped into 11 clusters with ≥ 5 patients, and most (82%, 109/133) were men who have sex with men (MSM) grouped into 8 clusters. The association with clusters was more frequent in Spanish (p = 0.02) men (p< 0.001), MSM (p<0.001) younger than 35 years (p = 0.001) and with a CD4+ T-cell count above 350 cells/ul (p<0.001). We estimated the date of HIV-1 subtype B regional epidemic diversification around 1970 (95% CI: 1965-1987), with an evolutionary rate of 2.4 (95%CI: 1.7-3.1) x 10-3 substitutions/site/year. Most clusters originated in the 1990s in MSMs. We observed exponential subtype B HIV-1 growth in 1980-1990 and 2005-2008. The most significant migration routes for subtype B went from inland cities to seaside locations.CONCLUSIONSWe provide the first data on the phylodynamic and phylogeographic profiles of HIV-1 subtype B in south Spain. Our findings of transmission clustering among MSMs should alert healthcare managers to enhance preventive measures in this risk group in order to prevent future outbreaks.
PB Public Library of Science
YR 2016
FD 2016-12-21
LK http://hdl.handle.net/10668/2599
UL http://hdl.handle.net/10668/2599
LA en
NO Pérez-Parra S, Chueca N, Álvarez M, Pasquau J, Omar M, Collado A, et al. Phylodynamic and Phylogeographic Profiles of Subtype B HIV-1 Epidemics in South Spain. PLoS ONE. 2016; 11(12):e0168099
NO Journal Article;
DS RISalud
RD Apr 6, 2025