RT Journal Article
T1 Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up.
A1 Soler, Maria
A1 Estevez, M-Carmen
A1 Moreno, Maria de Lourdes
A1 Cebolla, Angel
A1 Lechuga, Laura M
K1 33-mer gliadin peptide
K1 Celiac disease
K1 Gluten detection
K1 Gluten immunogenic peptides
K1 POC device
K1 Surface plasmon resonance
AB Motivated by the necessity of new and efficient methods for dietary gluten control of celiac patients, we have developed a simple and highly sensitive SPR biosensor for the detection of gluten peptides in urine. The sensing methodology enables rapid and label-free quantification of the gluten immunogenic peptides (GIP) by using G12 mAb. The overall performance of the biosensor has been in-depth optimized and evaluated in terms of sensitivity, selectivity and reproducibility, reaching a limit of detection of 0.33 ng mL(-1). Besides, the robustness and stability of the methodology permit the continuous use of the biosensor for more than 100 cycles with excellent repeatability. Special efforts have been focused on preventing and minimizing possible interferences coming from urine matrix enabling a direct analysis in this fluid without requiring extraction or purification procedures. Our SPR biosensor has proven to detect and identify gluten consumption by evaluating urine samples from healthy and celiac individuals with different dietary gluten conditions. This novel biosensor methodology represents a novel approach to quantify the digested gluten peptides in human urine with outstanding sensitivity in a rapid and non-invasive manner. Our technique should be considered as a promising opportunity to develop Point-of-Care (POC) devices for an efficient, simple and accurate gluten free diet (GFD) monitoring as well as therapy follow-up of celiac disease patients.
YR 2015
FD 2015-12-03
LK http://hdl.handle.net/10668/9681
UL http://hdl.handle.net/10668/9681
LA en
DS RISalud
RD Apr 6, 2025