RT Journal Article
T1 Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.
A1 Silva, Sonia
A1 Altmannova, Veronika
A1 Luke-Glaser, Sarah
A1 Henriksen, Peter
A1 Gallina, Irene
A1 Yang, Xuejiao
A1 Choudhary, Chunaram
A1 Luke, Brian
A1 Krejci, Lumir
A1 Lisby, Michael
K1 DNA repair
K1 Mph1
K1 Mte1
K1 genome integrity
K1 homologous recombination
K1 telomere maintenance
AB Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.
YR 2016
FD 2016-03-10
LK http://hdl.handle.net/10668/9907
UL http://hdl.handle.net/10668/9907
LA en
DS RISalud
RD Apr 6, 2025