RT Journal Article
T1 Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen.
A1 San Segundo-Acosta, Pablo
A1 Oeo-Santos, Carmen
A1 Benedé, Sara
A1 de Los Ríos, Vivian
A1 Navas, Ana
A1 Ruiz-Leon, Berta
A1 Moreno, Carmen
A1 Pastor-Vargas, Carlos
A1 Jurado, Aurora
A1 Villalba, Mayte
A1 Barderas, Rodrigo
K1 allergen
K1 allergenome
K1 cross-reactivity
K1 cyclophilin
K1 in-depth proteomics
K1 olive pollen proteome
AB Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree (Olea europaea L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl cis-trans isomerase, cyclophilin, produced in Escherichia coli, was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal, and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food, and animal extracts.
YR 2019
FD 2019-06-27
LK http://hdl.handle.net/10668/14103
UL http://hdl.handle.net/10668/14103
LA en
DS RISalud
RD Apr 11, 2025