RT Journal Article
T1 Crosstalk between chromatin structure, cohesin activity and transcription
A1 Maya-Miles, Douglas
A1 Andújar, Eloísa
A1 Pérez-Alegre, Mónica
A1 Murillo-Pineda, Marina
A1 Barrientos-Moreno, Marta
A1 Cabello-Lobato, María J.
A1 Gómez-Marín, Elena
A1 Morillo-Huesca, Macarena
A1 Prado, Félix
K1 Chromatin
K1 Cohesin
K1 Transcription
K1 Scc1
K1 Cromatina
K1 Transcripción genética
AB Background: A complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism.Results: We have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the - 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregulated genes are enriched in promoters with short or no nucleosome-free region (NFR) and a fragile "nucleosome - 1/RSC complex" particle. These results, together with a preferential increase in the occupancy of nucleosome - 1 of these genes, suggest that cohesins promote transcription activation by helping RSC to form the NFR. In sharp contrast, the scc1-73 upregulated genes are enriched in promoters with an "open" chromatin structure and are mostly at cohesin-enriched regions, suggesting that a local accumulation of cohesins might help to inhibit transcription. On the other hand, a dramatic loss of chromatin integrity by histone depletion during DNA replication has a moderate effect on the accumulation and distribution of cohesin peaks along the genome.Conclusions: Our analyses of the interplay between chromatin integrity and cohesin activity suggest that cohesins play a major role in transcription regulation, which is associated with specific chromatin architecture and cohesin-mediated nucleosome alterations of the regulated promoters. In contrast, chromatin integrity plays only a minor role in the binding and distribution of cohesins.
PB BioMed Central Ltd
YR 2019
FD 2019-07-22
LK http://hdl.handle.net/10668/3165
UL http://hdl.handle.net/10668/3165
LA en
NO Maya-Miles D, Andújar E, Pérez-Alegre M, Murillo-Pineda M, Barrientos-Moreno M, Cabello-Lobato MJ, et al. Crosstalk between chromatin structure, cohesin activity and transcription. Epigenetics Chromatin. 2019 Jul 22;12(1):47.
DS RISalud
RD Apr 5, 2025