RT Journal Article
T1 The Common Mosquito (Culex pipiens) Does Not Seem to Be a Competent Vector for Hepatitis E Virus Genotype 3
A1 Frias, Mario
A1 Casades-Marti, Laia
A1 Risalde, Maria A.
A1 Lopez-Lopez, Pedro
A1 Cuadrado-Matias, Raul
A1 Rivero-Juarez, Antonio
A1 Rivero, Antonio
A1 Ruiz-Fons, Francisco
K1 Vector competence
K1 HEV
K1 Mosquito
K1 Culex
K1 Experimental infection
AB An experimental infection approach was used to estimate the competence of the common mosquito, Culex pipiens, for hepatitis E virus replication and transmission, using an isolate of hepatitis E virus genotype 3 of human origin in varying infectious doses. The experimental approach was carried out in biosafety level 2 conditions on three batches of 120 Cx. pipiens females, each using an artificial feeding system containing the virus in aliquots of fresh avian blood. Mosquitoes from each batch were collected 1, 7, 14, and 21 days post-infection (dpi) and dissected. The proboscis was subjected to forced excretion of saliva to estimate potential virus transmission. HEV RNA presence in abdomen, thorax, and saliva samples was analyzed by PCR at the selected post-infection times. HEV RNA was detected in the abdomens of Cx. pipiens females collected 1 dpi in the two experimentally-infected batches, but not in the saliva or thorax. None of the samples collected 7-21 dpi were positive. Our results show that Cx. pipiens is not a competent vector for HEV, at least for zoonotic genotype 3.
PB Frontiers Research Foundation
YR 2022
FD 2022-03-31
LK http://hdl.handle.net/10668/20748
UL http://hdl.handle.net/10668/20748
LA en
NO Frías M, Casades-Martí L, Risalde MÁ, López-López P, Cuadrado-Matías R, Rivero-Juárez A, et al. The Common Mosquito (Culex pipiens) Does Not Seem to Be a Competent Vector for Hepatitis E Virus Genotype 3. Front Vet Sci. 2022 Apr 26;9:874030
DS RISalud
RD Apr 6, 2025