RT Journal Article
T1 Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis.
A1 Ruiz, Pilar
A1 Causse, Manuel
A1 Vaquero, Manuel
A1 Gutierrez, Juan Bautista
A1 Casal, Manuel
K1 Abbott RealTime MTB RIF/INH Resistance assay
K1 M. tuberculosis
K1 mutations
AB A new automated real-time PCR assay for the detection of rifampicin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB) was evaluated. A total of 163 clinical samples (128 pulmonary and 35 extra-pulmonary) were processed using four PCR assay kits: Abbott RealTime MTB RIF/INH, Genotype MTBDRplus, Xpert/MTB RIF, and Anyplex MTB/MDR. The results of phenotypic drug-susceptibility testing using BACTECMGIT 960 were used as reference. The sensitivity and specificity of the new Abbott RealTime MTB RIF/INH assay in comparison with phenotypic testing was 96.3% (95%CI 87.32%-100%) for RIF and 100% (95%CI 99.3%-100%) for INH; the sensitivity was 78.8% (95%CI 66.8%-90.9%) and the specificity was 100% (95%CI 98.9%-100%). The Abbott RealTime MTB RIF/INH test could be a valid method for detecting the most common mutations in strains resistant to RIF and INH.
SN 1178-6973
YR 2017
FD 2017-12-06
LK https://hdl.handle.net/10668/25740
UL https://hdl.handle.net/10668/25740
LA en
DS RISalud
RD Apr 11, 2025