RT Journal Article
T1 EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells.
A1 Santiago-Fernandez, Concepcion
A1 Rodriguez-Diaz, Cristina
A1 Ho-Plagaro, Ailec
A1 Gutierrez-Repiso, Carolina
A1 Oliva-Olivera, Wilfredo
A1 Martin-Reyes, Flores
A1 Mela, Virginia
A1 Bautista, Rocio
A1 Tome, Monicas
A1 Gomez-Maldonado, Josefa
A1 Tinahones, Francisco J
A1 Garcia-Fuentes, Eduardo
A1 Garrido-Sanchez, Lourdes
K1 HUVECs
K1 Extra-virgin olive oil
K1 Extracellular vesicles (EVs)
K1 miRNAs
K1 Smooth muscle cells (SMCs)
K1 Sunflower oil
AB Little is known about the effect of extra virgin olive (EVOO) and sunflower oil (SO) on the composition of extracellular vesicles (EVs) secreted by endothelial cells and the effects of these EVs on smooth muscle cells (SMCs). These cells play an important role in the development of atherosclerosis. We evaluated the effects of endothelial cells-derived EVs incubated with triglyceride-rich lipoproteins obtained after a high-fat meal with EVOO (EVOO-EVs) and SO (SO-EVs), on the transcriptomic profile of SMCs. We found 41 upregulated and 19 downregulated differentially expressed (DE)-miRNAs in EVOO-EVs. Afterwards, SMCs were incubated with EVOO-EVs and SO-EVs. SMCs incubated with SO-EVs showed a greater number of DE-mRNA involved in pathways related to cancer, focal adhesion, regulation of actin cytoskeleton, and MAPK, toll-like receptor, chemokine and Wnt signaling pathways than in SMCs incubated with EVOO-EVs. These DE-mRNAs were involved in biological processes related to the response to endogenous stimulus, cell motility, regulation of intracellular signal transduction and cell population proliferation. EVOO and SO can differently modify the miRNA composition of HUVEC-derived EVs. These EVs can regulate the SMCs transcriptomic profile, with SO-EVs promoting a profile more closely linked to the development of atherosclerosis than EVOO-EVs.
PB Frontiers Research Foundation
SN 2296-861X
YR 2022
FD 2022-04-12
LK http://hdl.handle.net/10668/20662
UL http://hdl.handle.net/10668/20662
LA en
NO Santiago-Fernandez C, Rodríguez-Díaz C, Ho-Plagaro A, Gutierrez-Repiso C, Oliva-Olivera W, Martin-Reyes F, et al. EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells. Front Nutr. 2022 Apr 12;9:867745
DS RISalud
RD Aug 30, 2025