RT Journal Article
T1 Regulation of transcription elongation in response to osmostress.
A1 Silva, Andrea
A1 Cavero, Santiago
A1 Begley, Victoria
A1 Solé, Carme
A1 Böttcher, René
A1 Chávez, Sebastián
A1 Posas, Francesc
A1 de Nadal, Eulàlia
AB Cells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed to osmostress. Besides being associated with transcription factors bound at target promoters to stimulate transcriptional initiation, activated Hog1 behaves as a transcriptional elongation factor that is selective for stress-responsive genes. Here, we provide insights into how this signaling kinase functions in transcription elongation. Hog1 phosphorylates the Spt4 elongation factor at Thr42 and Ser43 and such phosphorylations are essential for the overall transcriptional response upon osmostress. The phosphorylation of Spt4 by Hog1 regulates RNA polymerase II processivity at stress-responsive genes, which is critical for cell survival under high osmostress conditions. Thus, the direct regulation of Spt4 upon environmental insults serves to stimulate RNA Pol II elongation efficiency.
YR 2017
FD 2017-11-20
LK http://hdl.handle.net/10668/11818
UL http://hdl.handle.net/10668/11818
LA en
DS RISalud
RD Apr 10, 2025