RT Journal Article
T1 Measuring Vitamin D-3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals
A1 de Los Santos Castillo-Peinado, Laura
A1 Calderon-Santiago, Monica
A1 Dulcinea Herrera-Martinez, Aura
A1 Leon-Idougourram, Soraya
A1 Angeles Galvez-Moreno, Maria
A1 Luis Sanchez-Cano, Rafael
A1 Bouillon, Roger
A1 Manuel Quesada-Gomez, Jose
A1 Priego-Capote, Feliciano
K1 Vitamin D deficiency
K1 Vitamin D-3
K1 1,25-dihydroxyvitamin D-3
K1 24,25-dihydroxyvitamin D-3
K1 1,24,25-trihydroxyvitamin D-3
K1 SPE-LC-MS/MS
K1 Plasma
AB The main branch of vitamin D-3 metabolism involves several hydroxylation reactions to obtain mono-, di- and trihydroxylated metabolites, including the circulating and active forms-25(OH)D-3 and 1,25(OH)(2)D-3, respectively. However, most clinical trials strictly target the determination of 25(OH)D-3 to offer a view of the metabolic status of vitamin D-3. Due to the growing interest in expanding this restricted view, we have developed a method for measuring vitamin D-3 metabolism by determination of vitamin D-3, 25(OH)D-3, 24,25(OH)(2)D-3, 1,25(OH)(2)D-3 and 1,24,25(OH)(3)D-3 in human plasma. The method was based on SPE-LC-MS/MS with a large volume injection of human plasma (240 mu L). Detection of di- and trihydroxymetabolites, found at the picogram per milliliter level, was attained by the combined action of high preconcentration and clean-up effects. The method allows obtaining information about ratios such as the known vitamin D metabolite ratio (24,25(OH)(2)D-3/25(OH)D-3), which can provide complementary views of vitamin D-3 metabolic status. The method was applied to a cohort of obese patients and a reference cohort of healthy volunteers to find metabolic correlations between target analytes as well as differences as a function of vitamin D levels within and between cohorts.
PB MDPI
YR 2022
FD 2022-05-31
LK http://hdl.handle.net/10668/21575
UL http://hdl.handle.net/10668/21575
LA en
NO De los Santos Castillo-Peinado L, Calderón-Santiago M, Herrera-Martínez AD, León-Idougourram S, Gálvez-Moreno MÁ, Sánchez-Cano RL, et al. Measuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals. Separations [Internet]. 3 de junio de 2022;9(6):141
DS RISalud
RD Apr 8, 2025