RT Journal Article
T1 Histological and biochemical evaluation of plasma rich in growth factors treatment for grade II muscle injuries in sheep.
A1 Aguilar-Garcia, Daniel
A1 Fernandez-Sarmiento, J Andres
A1 Del Mar Granados Machuca, Maria
A1 Rodriguez, Juan Morgaz
A1 Rascon, Pilar Muñoz
A1 Calvo, Rocio Navarrete
A1 Ruiz, Yolanda Millan
A1 Poveda, Jose Maria Carrillo
A1 Castañeda, Juan Muñoz
A1 Bertomeu, Ramon Cugat
A1 Dominguez Perez, Juan Manuel
K1 Muscle injury
K1 Muscle repair
K1 Plasma rich in growth factors
K1 Platelet-rich plasma
AB The purpose of this study was to perform a histological and biochemical evaluation of the influence of plasma rich in growth factors (PRGF) on muscle regeneration process after a surgically induced grade II muscle laceration. A randomized, single blind, controlled experimental research was conducted including twenty-one adult healthy sheep, randomly divided in three groups (n = 7). A grade II surgical section was performed in the biceps femoris muscle of both hindlimbs. After two days (basal time), intralesional infiltration of autologous PRGF or Saline solution was randomly administered in both hindlimbs. Treatment was repeated once a week. Animal groups were euthanized at 1 (T1), 2 (T2) or 4 (T4) weeks. Histological assessment showed that PRGF intralesional injection induced a significant decrease of inflammatory cells density, significant higher centrally nucleated fibers percentage and significantly smaller fibrotic areas compared to Saline-treated muscles at T1, T2 and T4. Also, lower vascular density, with lower capillaries cross-sectional area, in PRGF group compared to Saline was observed. Biochemical analysis revealed a significant higher expression level of MYOD1, MYF5 and MYOG genes in PRGF groups at T1 compared to Saline treated muscles. At ultrastructural level, PRGF groups presented scarce edema and loss of connective tissue structure, as well as higher mitochondrial density adequately associated to the sarcomere unit in contrast to the Saline group. In conclusion, histological, biochemical, and ultrastructural results showed that PRGF treatment improved muscle regeneration process leading to more mature histological aspect in newly formed muscle tissue after a surgically induced grade II muscle injury.
PB BioMed Central
YR 2022
FD 2022-10-17
LK http://hdl.handle.net/10668/20311
UL http://hdl.handle.net/10668/20311
LA en
NO Aguilar-García D, Fernández-Sarmiento JA, Del Mar Granados Machuca M, Rodríguez JM, Rascón PM, Calvo RN, et al. Histological and biochemical evaluation of plasma rich in growth factors treatment for grade II muscle injuries in sheep. BMC Vet Res. 2022 Nov 12;18(1):400
DS RISalud
RD Apr 6, 2025