RT Journal Article
T1 Intrapopulation variability in mutator prevalence among urinary tract infection isolates of Escherichia coli
A1 Couce, A.
A1 Alonso-Rodriguez, N.
A1 Costas, C.
A1 Oliver, A.
A1 Blazquez, J.
K1 Antibiotic resistance
K1 hypermutability
K1 mutator
K1 mutL
K1 mutY
K1 Cystic-fibrosis
K1 Mismatch repair
K1 High-frequency
K1 Resistance
K1 Strains
K1 Emergence
K1 Evolution
K1 Bacteria
K1 Rates
K1 Populations
AB Bacteria with elevated mutation rates represent a risk factor for treatment failure and are often found with high frequency in clinical isolates from different sources. How this frequency reflects the among-population and within-population proportion of hypermutators is unknown, despite its importance to the choice of antibiotic therapies that minimize the likelihood of resistance development. Here we screened for hypermutators among the urine of 80 patients with urinary tract infections, at an unprecedented resolution of 24 isolates per sample. We found hypermutators in four patients (5%), at frequencies ranging from 4.2% to 62.5%. Molecular characterization revealed alterations in the oxidized guanine (GO) and methly-directed mistmatch repair (MMR) systems as the genetic basis of hypermutability. These observations suggest that mutators may be present in more patients than previously anticipated, at frequencies that are difficult to detect but still sufficient to impact on adaptation to antibiotics or the host environment. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
PB Elsevier sci ltd
SN 1198-743X
YR 2016
FD 2016-06-01
LK http://hdl.handle.net/10668/18747
UL http://hdl.handle.net/10668/18747
LA en
DS RISalud
RD Apr 10, 2025