RT Journal Article
T1 Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p.
A1 Bartolomeu, Ariane Rocha
A1 Romualdo, Guilherme Ribeiro
A1 Lison, Carmen Griñan
A1 Besharat, Zein Mersini
A1 Corrales, Juan Antonio Marchal
A1 Garcia Chaves, Maria Angel
A1 Barbisan, Luis Fernando
K1 caffeine
K1 chemically induced colon carcinogenesis
K1 chlorogenic acid
K1 miRNA expression
K1 mice
AB Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC.
PB MDPI AG
YR 2022
FD 2022-05-28
LK http://hdl.handle.net/10668/21162
UL http://hdl.handle.net/10668/21162
LA en
NO Bartolomeu AR, Romualdo GR, Lisón CG, Besharat ZM, Corrales JAM, Garcia Chaves MÁ, et al. Barbisan LF. Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p. Int J Mol Sci. 2022 Jun 4;23(11):6292.
DS RISalud
RD Apr 9, 2025