RT Journal Article
T1 Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.
A1 Morata, Pilar
A1 Queipo-Ortuño, María Isabel
A1 de Dios Colmenero, Juan de Dios
K1 España
K1 Brucelosis
K1 ADN bacteriano
K1 Electroforesis en gel de agar
K1 Leucocitos
K1 Reacción en cadena de la polimerasa
AB We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.
PB American Society for Microbiology
SN 0095-1137
YR 1998
FD 1998-09
LK http://hdl.handle.net/10668/2146
UL http://hdl.handle.net/10668/2146
LA en
NO Morata P, Queipo-Ortuño MI, Colmenero J de D. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998; 36(9):2443-6
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Jul 16, 2025