RT Journal Article
T1 Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts.
A1 Toledano-Osorio, Manuel
A1 Manzano-Moreno, Francisco J
A1 Toledano, Manuel
A1 Medina-Castillo, Antonio L
A1 Costela-Ruiz, Victor J
A1 Ruiz, Concepción
A1 Osorio, Raquel
K1 CD markers
K1 antigenic phenotype
K1 doxycycline
K1 membrane
K1 osteoblasts
AB Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts' proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p
YR 2021
FD 2021-03-28
LK https://hdl.handle.net/10668/28108
UL https://hdl.handle.net/10668/28108
LA en
DS RISalud
RD Apr 17, 2025