RT Journal Article
T1 Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept.
A1 Gomez-Gonzalez, Emilio
A1 Barriga-Rivera, Alejandro
A1 Fernandez-Muñoz, Beatriz
A1 Navas-Garcia, Jose Manuel
A1 Fernandez-Lizaranzu, Isabel
A1 Munoz-Gonzalez, Francisco Javier
A1 Parrilla-Giraldez, Ruben
A1 Requena-Lancharro, Desiree
A1 Gil-Gamboa, Pedro
A1 Rosell-Valle, Cristina
A1 Gomez-Gonzalez, Carmen
A1 Mayorga-Buiza, Maria Jose
A1 Martin-Lopez, Maria
A1 Muñoz, Olga
A1 Gomez-Martin, Juan Carlos
A1 Relimpio-Lopez, Maria Isabel
A1 Aceituno-Castro, Jesus
A1 Perales-Esteve, Manuel A
A1 Puppo-Moreno, Antonio
A1 Garcia-Cozar, Francisco Jose
A1 Olvera-Collantes, Lucia
A1 Gomez-Diaz, Raquel
A1 de Los Santos-Trigo, Silvia
A1 Huguet-Carrasco, Monserrat
A1 Rey, Manuel
A1 Gomez, Emilia
A1 Sanchez-Pernaute, Rosario
A1 Padillo-Ruiz, Javier
A1 Marquez-Rivas, Javier
K1 Red Andaluza de Diseño y Traslación de Terapias Avanzadas
AB Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.
PB Nature Publishing Group
YR 2022
FD 2022-01-28
LK http://hdl.handle.net/10668/19585
UL http://hdl.handle.net/10668/19585
LA en
NO Gomez-Gonzalez E, Barriga-Rivera A, Fernandez-Muñoz B, Navas-Garcia JM, Fernandez-Lizaranzu I, Munoz-Gonzalez FJ, et al. Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept. Sci Rep. 2022 Feb 18;12(1):2356
NO Te authors would like to gratefully acknowledge the assistance of the members of the Explosive Ordnance Disposal—Chemical, Biological, Radiological & Nuclear (EOD-CBRN) Group of the Spanish National Police,whose identities cannot be disclosed, and who are represented here by JMNG. Te authors also thank Manuel Guerrero-Claro for his technical assistance with the implementation of the neural networks, and the companies CAMBRICO BIOTECH (Sevilla, Spain), CER “Dr. Gregorio Medina Blanco” (Espartinas, Sevilla, Spain) and SAMU (Sevilla, Spain) for their collaboration in the collection of human samples. Tis research was funded byGrants Number COV20-00080 and COV20-00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the Institute of Health ‘Carlos III’, Spanish Ministry of Scienceand Innovation, and by Grant Number EQC2019-006240-P funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. ABR was supported by Grant Number RTI2018-094465-J-I00 funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. Tis work has been supported by the European Commission through the Joint Research Center (JRC) HUMAINT project.
DS RISalud
RD Apr 10, 2025