%0 Journal Article
%A Gomez-Gonzalez, Emilio
%A Barriga-Rivera, Alejandro
%A Fernandez-Muñoz, Beatriz
%A Navas-Garcia, Jose Manuel
%A Fernandez-Lizaranzu, Isabel
%A Munoz-Gonzalez, Francisco Javier
%A Parrilla-Giraldez, Ruben
%A Requena-Lancharro, Desiree
%A Gil-Gamboa, Pedro
%A Rosell-Valle, Cristina
%A Gomez-Gonzalez, Carmen
%A Mayorga-Buiza, Maria Jose
%A Martin-Lopez, Maria
%A Muñoz, Olga
%A Gomez-Martin, Juan Carlos
%A Relimpio-Lopez, Maria Isabel
%A Aceituno-Castro, Jesus
%A Perales-Esteve, Manuel A
%A Puppo-Moreno, Antonio
%A Garcia-Cozar, Francisco Jose
%A Olvera-Collantes, Lucia
%A Gomez-Diaz, Raquel
%A de Los Santos-Trigo, Silvia
%A Huguet-Carrasco, Monserrat
%A Rey, Manuel
%A Gomez, Emilia
%A Sanchez-Pernaute, Rosario
%A Padillo-Ruiz, Javier
%A Marquez-Rivas, Javier
%T Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept.
%D 2022
%U http://hdl.handle.net/10668/19585
%X Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.
%K Red Andaluza de Diseño y Traslación de Terapias Avanzadas
%~