RT Journal Article
T1 Redox status in the sentinel lymph node of women with breast cancer.
A1 Ramírez-Expósito, María Jesús
A1 Urbano-Polo, Nieves
A1 Dueñas, Basilio
A1 Navarro-Cecilia, Joaquín
A1 Ramírez-Tortosa, César
A1 Martín-Salvago, María Dolores
A1 Martínez-Martos, José Manuel
K1 Breast cancer
K1 GSH
K1 OSNA
K1 catalase
K1 glutathione peroxidase
K1 sentinel lymph node
K1 superoxide dismutase
K1 total antioxidant capacity
AB Lymphatic metastasis is regulated in multiple steps including the transit of tumor cells via the lymphatic vessels and the successful seeding in draining lymph nodes. Thus, several molecular signals and cellular changes must be involved in this complex process to facilitate tumor cell entry, colonization, and survival in the lymph node. To our knowledge, the present work explores, for the first time in the literature, the redox status (oxidative stress parameters and enzymatic and non-enzymatic antioxidant defense systems) in the sentinel lymph node (SLN) of women with breast cancer. SLNs from 75 women with breast cancer were identified using the one-step nucleic acid amplification (OSNA) method as negative (n = 43), with micrometastases (n = 13), or with macrometastases (n = 19). It will allow us to gain knowledge about the pro-oxidant/antioxidant mechanisms involved in the processes of distant metastases in breast cancer and also to assess whether these parameters may be alternative techniques for staging. We found different levels of lipid peroxidation in SLNs with micrometastases (increased) and macrometastases (decreased), a decrease in carbonyl group content in SLNs with macrometastases only, and an increase in total antioxidant capacity (TAC) in SNLs with micrometastases and macrometastases. A decrease in the levels of reduced glutathione (GSH) also appears in the SLNs with macrometastases only. Finally, we show increased levels of superoxide dismutase (SOD) and catalase (CAT) activity in SLNs with micrometastases and macrometastases, and decreased levels of glutathione peroxidase (GPx) activity in SNLs with macrometastases but not with micrometastases. Redox status of lymph node microenvironment participates in the progression of metastatic breast cancer.
YR 2017
FD 2017-12-21
LK http://hdl.handle.net/10668/11933
UL http://hdl.handle.net/10668/11933
LA en
DS RISalud
RD Apr 6, 2025