RT Journal Article
T1 Development of an injectable alginate-collagen hydrogel for cardiac delivery of extracellular vesicles.
A1 Gil-Cabrerizo, Paula
A1 Saludas, Laura
A1 Prósper, Felipe
A1 Abizanda, Gloria
A1 Echanove-González de Anleo, Miguel
A1 Ruiz-Villalba, Adrián
A1 Garbayo, Elisa
A1 Blanco-Prieto, María J
K1 Alginate
K1 Collagen
K1 Extracellular vesicles
K1 Hydrogel
K1 Myocardial infarction
AB Extracellular vesicles (EVs) are nanosized particles with attractive therapeutic potential for cardiac repair. However, low retention and stability after systemic administration limit their clinical translation. As an alternative, the combination of EVs with biomaterial-based hydrogels (HGs) is being investigated to increase their exposure in the myocardium and achieve an optimal therapeutic effect. In this study, we developed and characterized a novel injectable in-situ forming HG based on alginate and collagen as a cardiac delivery vehicle for EVs. Different concentrations of alginate and collagen crosslinked with calcium gluconate were tested. Based on injectability studies, 1% alginate, 0.5 mg/mL collagen and 0.25% calcium gluconate HG was selected as the idoneous combination for cardiac administration using catheter-based systems. Rheological examination revealed that the HG possessed an internal gel structure, weak mechanical properties and low viscosity, facilitating an easy administration. In addition, EVs were successfully incorporated and homogeneously distributed in the HG. After administration in a rat model of myocardial infarction, the HG showed long-term retention in the heart and allowed for a sustained release of EVs for at least 7 days. Thus, the combination of HGs and EVs represents a promising therapeutic strategy for myocardial repair. Besides EVs delivery, the developed HG could represent a useful platform for cardiac delivery of multiple therapeutic agents.
YR 2022
FD 2022-11-01
LK http://hdl.handle.net/10668/22271
UL http://hdl.handle.net/10668/22271
LA en
DS RISalud
RD Apr 11, 2025