RT Journal Article
T1 Development of an injectable alginate-collagen hydrogel for cardiac delivery of extracellular vesicles.
A1 Gil-Cabrerizo, Paula
A1 Saludas, Laura
A1 Prosper, Felipe
A1 Abizanda, Gloria
A1 Echanove-Gonzalez-de-Anleo, Miguel
A1 Ruiz-Villalba, Adrian
A1 Garbayo, Elisa
A1 Blanco-Prieto, Maria J
K1 Alginate
K1 Collagen
K1 Extracellular vesicles
K1 Hydrogel
K1 Myocardial infarction
K1 Centro Andaluz de Nanomedicina y Biotecnología (BIONAND)
AB Extracellular vesicles (EVs) are nanosized particles with attractive therapeutic potential for cardiac repair. However, low retention and stability after systemic administration limit their clinical translation. As an alternative, the combination of EVs with biomaterial-based hydrogels (HGs) is being investigated to increase their exposure in the myocardium and achieve an optimal therapeutic effect. In this study, we developed and characterized a novel injectable in-situ forming HG based on alginate and collagen as a cardiac delivery vehicle for EVs. Different concentrations of alginate and collagen crosslinked with calcium gluconate were tested. Based on injectability studies, 1% alginate, 0.5 mg/mL collagen and 0.25% calcium gluconate HG was selected as the idoneous combination for cardiac administration using catheter-based systems. Rheological examination revealed that the HG possessed an internal gel structure, weak mechanical properties and low viscosity, facilitating an easy administration. In addition, EVs were successfully incorporated and homogeneously distributed in the HG. After administration in a rat model of myocardial infarction, the HG showed long-term retention in the heart and allowed for a sustained release of EVs for at least 7 days. Thus, the combination of HGs and EVs represents a promising therapeutic strategy for myocardial repair. Besides EVs delivery, the developed HG could represent a useful platform for cardiac delivery of multiple therapeutic agents.
PB Elsevier BV
YR 2022
FD 2022-12-15
LK http://hdl.handle.net/10668/22271
UL http://hdl.handle.net/10668/22271
LA en
NO Gil-Cabrerizo P, Saludas L, Prósper F, Abizanda G, Echanove-González de Anleo M, Ruiz-Villalba A, et al. Development of an injectable alginate-collagen hydrogel for cardiac delivery of extracellular vesicles. Int J Pharm. 2022 Dec 15;629:122356.
NO This work was supported by the Spanish Ministry of Economy and Competitiveness (SAF2017-83734-R). Adrian Ruiz-Villalba is supported by funds from University of Málaga (ayuda B1 – Plan Propio UMA) and Ministry of Science and innovation-State Research Agency/ PID2020-119430RJ-I00. Elisa Garbayo is supported by an FSE/Ministry of Science and innovation-State Research Agency/ RYC2018-025897-I.
DS RISalud
RD Oct 30, 2025