RT Journal Article
T1 Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery.
A1 Lebrón, José Antonio
A1 López-López, Manuel
A1 García-Calderón, Clara B
A1 V Rosado, Ivan
A1 Balestra, Fernando R
A1 Huertas, Pablo
A1 Rodik, Roman V
A1 Kalchenko, Vitaly I
A1 Bernal, Eva
A1 Moyá, María Luisa
A1 López-Cornejo, Pilar
A1 Ostos, Francisco J
K1 cationic calix[4]arenes
K1 doxorubicin
K1 encapsulation
K1 liposomes
K1 nucleic acids
K1 transfection efficiency
AB The formation of calixarene-based liposomes was investigated, and the characterization of these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the polar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formed with one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the results obtained, the liposomes formed with the least cytotoxic calixarene, (TEAC12)4, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that (TEAC12)4/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material, although the transfection efficiency substantially increases in the presence of an additional amount of DOPE as coadjuvant. On the other hand, the (TEAC12)4/DOPE liposomes present a high doxorubicin encapsulation efficiency, and a slow controlled release, which could diminish the side effects of the drug.
SN 1999-4923
YR 2021
FD 2021-08-12
LK https://hdl.handle.net/10668/28375
UL https://hdl.handle.net/10668/28375
LA en
DS RISalud
RD Apr 6, 2025