RT Journal Article
T1 Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis.
A1 Heinonen, Santtu
A1 Jartti, Tuomas
A1 Garcia, Carla
A1 Oliva, Silvia
A1 Smitherman, Cynthia
A1 Anguiano, Esperanza
A1 de-Steenhuijsen-Piters, Wouter A A
A1 Vuorinen, Tytti
A1 Ruuskanen, Olli
A1 Dimo, Blerta
A1 Suarez, Nicolas M
A1 Pascual, Virginia
A1 Ramilo, Octavio
A1 Mejias, Asuncion
K1 Asymptomatic
K1 Children
K1 Rhinovirus
K1 Transcriptional profiling
K1 Viral detection
AB Rhinoviruses (RVs) are a major cause of symptomatic respiratory tract infection in all age groups. However, RVs can frequently be detected in asymptomatic individuals. To evaluate the ability of host transcriptional profiling to differentiate between symptomatic RV infection and incidental detection in children. Previously healthy children younger than 2 years old (n = 151) were enrolled at four study sites and classified into four clinical groups: RV- healthy control subjects (n = 37), RV+ asymptomatic subjects (n = 14), RV+ outpatients (n = 30), and RV+ inpatients (n = 70). Host responses were analyzed using whole-blood RNA transcriptional profiles. RV infection induced a robust transcriptional signature, which was validated in three independent cohorts and by quantitative real-time polymerase chain reaction with high prediction accuracy. The immune profile of symptomatic RV infection was characterized by overexpression of innate immunity and underexpression of adaptive immunity genes, whereas negligible changes were observed in asymptomatic RV+ subjects. Unsupervised hierarchical clustering identified two main clusters of subjects. The first included 93% of healthy control subjects and 100% of asymptomatic RV+ subjects, and the second comprised 98% of RV+ inpatients and 88% of RV+ outpatients. Genomic scores of healthy control subjects and asymptomatic RV+ children were similar and significantly lower than those of RV+ inpatients and outpatients (P  Symptomatic RV infection induced a robust and reproducible transcriptional signature, whereas identification of RV in asymptomatic children was not associated with significant systemic transcriptional immune responses. Transcriptional profiling represents a useful tool to discriminate between active infection and incidental virus detection.
PB American Thoracic Society
YR 2015
FD 2015-11-13
LK http://hdl.handle.net/10668/9623
UL http://hdl.handle.net/10668/9623
LA en
NO Heinonen S, Jartti T, Garcia C, Oliva S, Smitherman C, Anguiano E, et al.  Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis. Am J Respir Crit Care Med. 2016 Apr 1;193(7):772-82
NO This work was supported in part by National Institute of Allergy and Infectious Diseases grant U19AI089987 (O. Ramilo and A.M.); Nationwide Children’sHospital intramural funds (A.M.); Academy of Finland grants 132595 and 114034 and Sigrid Juselius Foundation grants (T.J.); and nonfederal grants from theEuropean Society for Paediatric Infectious Diseases (ESPID Fellowship Award), the Finnish Medical Foundation, the Foundation for Pediatric Research, and theMaud Kuistila Memorial Foundation (S.H.).
DS RISalud
RD May 10, 2025