RT Journal Article
T1 Pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains.
A1 Plaza-Díaz, Julio
A1 Fernández-Caballero, Jose Ángel
A1 Chueca, Natalia
A1 García, Federico
A1 Gómez-Llorente, Carolina
A1 Sáez-Lara, María José
A1 Fontana, Luis
A1 Gil, Ángel
K1 Gut
K1 Healthy
K1 Adults
K1 High-throughput nucleotide sequencing
K1 Microbiota
K1 Probiotics
K1 ADN bacteriano
K1 Microbioma gastrointestinal
K1 Voluntarios sanos
K1 Secuenciación de nucleótidos de alto rendimiento
K1 Filogenia
K1 Probióticos
K1 ARN ribosómico 16S
K1 Análisis de secuencia de ADN
AB The colon microbiota plays a crucial role in human gastrointestinal health. Current attempts to manipulate the colon microbiota composition are aimed at finding remedies for various diseases. We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035). The goal of the present study was to analyze the compositions of the fecal microbiota of healthy adults who received one of these strains using high-throughput 16S ribosomal RNA gene sequencing. Bacteroides was the most abundant genus in the groups that received L. rhamnosus CNCM I-4036 or L. paracasei CNCM I-4034. The Shannon indices were significantly increased in these two groups. Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036. The initially different colon microbiota became homogeneous in the subjects who received L. rhamnosus CNCM I-4036. While some orders that were initially present disappeared after the administration of L. rhamnosus CNCM I-4036, other orders, such as Sphingobacteriales, Nitrospirales, Desulfobacterales, Thiotrichales, and Synergistetes, were detected after the intervention. In summary, our results show that the intake of these three bacterial strains induced changes in the colon microbiota.
PB MDPI
YR 2015
FD 2015-05-26
LK http://hdl.handle.net/10668/2346
UL http://hdl.handle.net/10668/2346
LA en
NO Plaza-Díaz J, Fernández-Caballero JÁ, Chueca N, García F, Gómez-Llorente C, Sáez-Lara MJ, et al. Pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains. Nutrients. 2015                         ; 7(6):3999-4015
NO Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 17, 2025